بررسی اثر هندسه‌ی داربست مهندسی بافت استخوان بر مدولاسیون مکانیکی رفتار لایه سلولی

نوع مقاله : مقاله پژوهشی

نویسندگان

دانشکده علوم و فنون نوین، دانشگاه تهران، تهران، ایران

چکیده

پیشرفت در روش‌های تولید افزایشی تاثیر چشمگیری در امکان کنترل و اصلاح طرح داخلی داربست استخوانی و ویژگی‌های آن گذاشته است. این امر موجب ارائه‌ی روش‌های نوین برای طراحی داربست‌های مهندسی بافت استخوان شده است. طراحی کامپیوتری داربست‌های مبتنی بر سطوح مینیمال مثلثاتی به دلیل نسبت سطح به حجم بالا که عاملی حیاتی در پژوهش‌های زیستی است، مطرح می‌باشد. از آنجایی که تحریک‌های مکانیکی اعمال شده در حین عبور سیال از داخل تخلخل‌های داربست، بر تکثیر، مهاجرت، تمایز و سرنوشت سلول‌های بنیادی مزانشیمی اثر دارد و این تحریک‌های مکانیکی خود متاثر از هندسه‌ی داخلی داربست هستند. در این پژوهش نگاهی دقیق‌تر به این موضوع افکنده می‌شود و با استفاده از ابزار دینامیک سیالات محاسباتی دو داربست مهندسی بافت استخوان مبتنی بر سطوح مینیمال مثلثاتی با نام‌های G و I از منظر مدولاسیون مکانیکی و برهمکنش با لایه‌ی سلولی به ضخامت 8/5 میکرومتر که نماینده‌ی تجمع سلولی روی داربست است، مورد بررسی قرار می‌گیرد. داربست G به دلیل هندسه‌ی داخلی مناسب و ایجاد توزیع تنش برشی مناسب روی لایه‌ی سلولی شرایط بهتری را برای کشت سلول و برهمکنش سیال-سازه ایجاد می‌کند. از سویی دیگر در داربست I نقاط مرده‌ای ایجاد می‌شود که یکنواختی سیگنال‌دهی در سطح آن را محدود می‌کند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Bone Tissue Engineering Scaffold Architecture on Mechanical Modulation of Cell Layer Behavior

نویسندگان [English]

  • Amirala Bakhshian Nik
  • Bahman Vahidi
MSc/University of Tehran
چکیده [English]

Advances in Additive Manufacturing (AM) techniques have made the design, control and modification of bone scaffolds inner architectures and their mechanical properties possible.  CAD bone scaffolds based on triply periodic minimal surfaces (TPMSs) have attracted attentions, due to their high surface area to volume ratio pore interconnectivity which enhance cell migration and attachment. The mechanical stimuli acting while fluid is flowing through scaffold pores can influence on proliferation, migration, differentiation and fate of mesenchymal stem cell. In the present study, the interaction between 2 TPMS-based bone scaffolds, termed G and I, with fluid in the presence 8.5 μm-cell layer (as mesenchymal stem cell accumulation) have been evaluated computationally. The results demonstrated that the scaffold G can modulate the cells more adequate due to producing homogenous distribution of mechanical stimuli comparing to scaffold I. The range of shear stress and von Mises stress for scaffold G are not wide which means the cells are sensing roughly the same mechanical stimuli. For both scaffolds in inlet velocities less than 50 μm/s, the magnitude of stresses is negligible. In addition, for scaffold I, there are dead zones which mechanical stimuli are approximately zero which prevents dynamic cell culture and homogenous signaling.

کلیدواژه‌ها [English]

  • Bone Tissue Engineering Scaffold
  • Computational Fluid Dynamics
  • Cell Layer
  • Mechanical Modulation
  • Mesenchymal Stem Cell

مراجع

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نشریه مهندسی مکانیک امیرکبیر
دوره 51، شماره 3
مرداد و شهریور 1398
صفحه 11-20

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