طراحی و مدل‌سازی یک ریزجداکننده دی الکتروفورزیس جدید دو طبقه مبتنی بر ریزسیالات‌ برای جداسازی کارآمد سلول‌های تومور گردشی

نوع مقاله : مقاله پژوهشی

نویسندگان

دانشکده مهندسی برق، دانشگاه بناب، بناب ، ایران

چکیده

جداسازی قابل اطمینان سلول‌های تومور گردشی از سلول‌های خونی برای تحلیل و پیش‌بینی زودهنگام سرطان امری حیاتی محسوب می‌شود. هدف از این پژوهش، جداسازی سلول‌های تومور گردشی و زیرگونه‌های سلول‌های سفید خون (گرانولوسیت‌ها و T-لنفوسیت‌ها) با به‌کارگیری نیروی دی الکتروفورزیس است. دی الکتروفورزیس به حرکت یک ذره خنثی اما قطبش‌پذیر مانند یک سلول، ویروس یا نانوذره در یک میدان الکتریکی ناهمگن اشاره دارد. دی الکتروفورزیس یک ابزار قدرتمند در ریزسیالات و دستگاه‌های آزمایشگاه-روی-یک-تراشه برای مرتب‌سازی سلولی است، مانند جداسازی سلول‌های سرطانی از سلول‌های خونی یا انواع مختلف باکتری. در این مطالعه، یک دستگاه ریزسیال‌ نوآورانه مجهز به الکترودهای حلقوی با ولتاژ پایین ۱ ولت برای کانال اصلی و 0/8 ولت برای کانال فرعی پیشنهاد شده که در فرکانس ۱۰۰ کیلوهرتز عمل می‌کند. استفاده از ولتاژ پایین، بقا و سلامت سلول‌های زیستی را تضمین می‌نماید که عاملی کلیدی در کاربردهای پزشکی به شمار می‌رود. نمودار‌های پتانسیل الکتریکی، سرعت، فشار و نیروی دی الکتروفورزیس اعمال شده بر سه نوع سلول مورد مطالعه از طریق شبیه‌سازی ارائه گردید. بازدهی ریزجداکننده پیشنهادی برای سلول‌های سرطانی حدود ۹۴٪ محاسبه شده است. در ادامه، با بهره‌گیری از روش المان محدود به عنوان یک رویکرد مقایسه ای، تأثیر تغییر ولتاژ الکترودهای کانال اصلی بر جداسازی کارآمد ذرات مورد بررسی و تحلیل قرار گرفت.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Design and Modeling of a Novel Two-stage Dielectrophoresis-Based Microfluidic Microseparator for Efficient Circulating Tumor Cell Separation

نویسندگان [English]

  • Reza Hadjiaghaie Vafaie
  • Elnaz Poorreza
Department of Electrical Engineering,- faculty Engineering- University of Bonab,- Bonab- Iran
چکیده [English]

The reliable separation of circulating tumor cells from blood cells is crucial for early cancer analysis and prediction. The aim of this research is the separation of circulating tumor cells and subtypes of white blood cells (Granulocytes and T-lymphocytes) by employing dielectrophoretic force. Dielectrophoresis refers to the movement of a neutral but polarizable particle, such as a cell, virus, or nanoparticle, in a non-uniform electric field. It is a powerful tool in microfluidics and lab-on-a-chip devices for cell sorting, such as separating cancer cells from blood cells or different types of bacteria. In this study, an innovative microfluidic device equipped with planar electrodes operating at a low voltage of 1 V for the main channel and 0.8 V for the second channel is proposed, which functions at a frequency of 100 kHz. The use of low voltage ensures the survival and health of biological cells, which is a key factor in medical applications. Plots of electrical potential, velocity, pressure, and the dielectrophoretic force applied to the three studied cell types were presented through simulations. The efficiency of the proposed microseparator for cancer cells was calculated to be approximately 94%. Subsequently, using the Finite Element Method as a comparative approach, the impact of changing the voltage of the main channel electrodes on the efficient separation of particles was investigated and analyzed.

کلیدواژه‌ها [English]

  • Microfluidics
  • Fluid Mechanics
  • Dielectrophoresis
  • Cancer Cell Separation
  • Simulation
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