Numerical Study on the Complete Separation of Blood Cells using the Integrated Dielectrophoretic-Photophoretic Method in a New Microchannel

Document Type : Research Article

Authors

1 Faculty of Mechanical Engineering, Tarbiat Modares University, Tehran, Iran

2 Faculty of Electrical & Computer Engineering, Tarbiat Modares University, Tehran, Iran

3 Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Abstract

In the present study, a numerical simulation was conducted to investigate the separation of blood cells using an integrated dielectrophoretic-photophoretic method in a new microfluidic device. In this simulation, the migration behavior of human blood cells under laser radiation with a wavelength of 522 nm and in the presence of fluid flow has been investigated. Studies show that the photophoretic migration of red cells under the irradiation of laser beam is higher than platelets and other blood cells so that the magnitude of the applied photoelectric force on the red blood cells has been calculated about nine times that of the white blood cells under the irradiation of laser beam of 50. In this separation using photophoretic forces, red blood cells were first separated from the platelets and white cells. Subsequently, using the hydrodynamic forces induced by the fluid on the particles and the dielectrophoretic forces, the separation of the platelets from the white blood cells was carried out in different branches of the microchannel. The proposed design, in addition to high separation efficiency, has a negligible cell loss, so that it can be used as an effective method in many diagnostic processes and medical applications.

Keywords

Main Subjects

References

[1] E. Bakker, M. Qattan, L. Mutti, C. Demonacos, M. Krstic-Demonacos, The role of microenvironment and immunity in drug response in leukemia, Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1863(3) (2016) 414-426.
[2] B. Paul, I.D. Nesbitt, Anaemia and blood transfusion, Surgery (Oxford), 31(2) (2013) 59-66.
[3] I. Rădulescu, D. Candea, A. Halanay, Optimal control analysis of a leukemia model under imatinib treatment, Mathematics and computers in Simulation, 121 (2016) 1-11.
[4] J.-L. Cheng, S.-F. Han, Y.-Q. Li, Y.-P. Chu, Y.-M. Sun, J.-F. Guo, An experimental study on RBC count and serum potassium concentration changes during compression transfusion of WBC-removal whole blood, Chinese Nursing Research, 2(2-3) (2015) 89-92.
[5] A. Ashkin, Atomic-beam deflection by resonance-radiation pressure, Physical Review Letters, 25(19) (1970) 1321.
[6] A. Ashkin, Forces of a single-beam gradient laser trap on a dielectric sphere in the ray optics regime, Biophysical journal, 61(2) (1992) 569-582.
[7] T. Imasaka, Y. Kawabata, T. Kaneta, Y. Ishidzu, Optical chromatography, Analytical Chemistry, 67(11) (1995) 1763-1765.
[8] A. Hirai, H. Monjushiro, H. Watarai, Laser photophoresis of a single droplet in oil in water emulsions, Langmuir, 12(23) (1996) 5570-5575.
[9] M. Zabetian, M.S. Saidi, M.B. Shafii, M.H. Saidi, Separation of microparticles suspended in a minichannel using laser radiation pressure, Applied Optics, 52(20) (2013) 4950-4958.
[10] H. Monjushiro, Y. Tanahashi, H. Watarai, Laser-photophoretic migration and fractionation of human blood cells, Analytica chimica acta, 777 (2013) 86-90.
[11] H. Monjushiro, A. Hirai, H. Watarai, Size dependence of laser-photophoretic efficiency of polystyrene microparticles in water, Langmuir, 16(22) (2000) 8539-8542.
[12] H. Monjushiro, K. Takeuchi, H. Watarai, Anomalous laser photophoretic behavior of photo-absorbing organic droplets in water, Chemistry letters, 31(8) (2002) 788-789.
[13] H. Monjushiro, M. Tanaka, H. Watarai, Periodic expansion-contraction motion of photoabsorbing organic droplets during laser photophoretic migration in water, Chemistry letters, 32(3) (2003) 254-255.
[14] S. Takatani, M.D. Graham, Theoretical analysis of diffuse reflectance from a two-layer tissue model, IEEE Transactions on Biomedical Engineering, (12) (1979) 656-664.
[15] M.P. Hughes, Strategies for dielectrophoretic separation in laboratory‐on‐a‐chip systems, Electrophoresis, 23(16) (2002) 2569-2582.
[16] B. Çetin, D. Li, Dielectrophoresis in microfluidics technology, Electrophoresis, 32(18) (2011) 2410-2427.
[17] M.S. Pommer, Y. Zhang, N. Keerthi, D. Chen, J.A. Thomson, C.D. Meinhart, H.T. Soh, Dielectrophoretic separation of platelets from diluted whole blood in microfluidic channels, Electrophoresis, 29(6) (2008) 1213-1218.
[18] K.-H. Han, A.B. Frazier, Lateral-driven continuous dielectrophoretic microseparators for blood cells suspended in a highly conductive medium, Lab on a Chip, 8(7) (2008) 1079-1086.
[19] S. Park, Y. Zhang, T.-H. Wang, S. Yang, Continuous dielectrophoretic bacterial separation and concentration from physiological media of high conductivity, Lab on a Chip, 11(17) (2011) 2893-2900.
[20] S. Dash, S. Mohanty, S. Pradhan, B. Mishra, CFD design of a microfluidic device for continuous dielectrophoretic separation of charged gold nanoparticles, Journal of the Taiwan Institute of Chemical Engineers, 58 (2016) 39-48.
[21] T. Braschler, N. Demierre, E. Nascimento, T. Silva, A.G. Oliva, P. Renaud, Continuous separation of cells by balanced dielectrophoretic forces at multiple frequencies, Lab on a Chip, 8(2) (2008) 280-286.
[22] B. Mathew, A. Alazzam, M. Abutayeh, A. Gawanmeh, S. Khashan, Modeling the trajectory of microparticles subjected to dielectrophoresis in a microfluidic device for field flow fractionation, Chemical Engineering Science, 138 (2015) 266-280.
[23] N. Piacentini, G. Mernier, R. Tornay, P. Renaud, Separation of platelets from other blood cells in continuous-flow by dielectrophoresis field-flow-fractionation, Biomicrofluidics, 5(3) (2011) 034122.
[24] S. Patel, D. Showers, P. Vedantam, T.-R. Tzeng, S. Qian, X. Xuan, Microfluidic separation of live and dead yeast cells using reservoir-based dielectrophoresis, Biomicrofluidics, 6(3) (2012) 034102.
[25] A. Kale, S. Patel, X. Xuan, Three-Dimensional Reservoir-Based Dielectrophoresis (rDEP) for Enhanced Particle Enrichment, Micromachines, 9(3) (2018) 123.
[26] J. Kadaksham, P. Singh, N. Aubry, Manipulation of particles using dielectrophoresis, Mechanics Research Communications, 33(1) (2006) 108-122.
[27] H. Bruus, Theoretical microfluidics, Oxford university press Oxford, 2008.
[28] Y.-S. Choi, K.-W. Seo, S.-J. Lee, Lateral and cross-lateral focusing of spherical particles in a square microchannel, Lab on a Chip, 11(3) (2011) 460-465.
[29] E. Evans, Y.-C. Fung, Improved measurements of the erythrocyte geometry, Microvascular research, 4(4) (1972) 335-347.
[30] P. Gascoyne, J. Satayavivad, M. Ruchirawat, Microfluidic approaches to malaria detection, Acta tropica, 89(3) (2004) 357-369.
[31] V. Nerguizian, A. Alazzam, D. Roman, I. Stiharu, M. Burnier Jr, Analytical solutions and validation of electric field and dielectrophoretic force in a bio‐microfluidic channel, Electrophoresis, 33(3) (2012) 426-435.
[32] J. Cottet, A. Kehren, S. Lasli, H. van Lintel, F. Buret, M. Frénéa‐Robin, P. Renaud, Dielectrophoresis‐assisted creation of cell aggregates under flow conditions using planar electrodes, Electrophoresis,  (2019).
[33] K. Tatsumi, K. Kawano, H. Okui, H. Shintani, K. Nakabe, Analysis and measurement of dielectrophoretic manipulation of particles and lymphocytes using rail-type electrodes, Medical engineering & physics, 38(1) (2016) 24-32.
[34] M. Egger, E. Donath, Electrorotation measurements of diamide-induced platelet activation changes, Biophysical journal, 68(1) (1995) 364-372.
 
Amirkabir Journal of Mechanical Engineering
Volume 53, Issue 1 (Special Issue)
April 2021
Pages 655-670

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