تولید میکرو قطرات در یک میکروکانال با استفاده از کنترل‌کننده تناسبی-انتگرال‌گیر-مشتق‌گیر: بررسی آزمایشگاهی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناسی ارشد، دانشکده مکانیک، دانشگاه صنعتی شاهرود،

2 صنعتی شاهرود

3 Shahrood University of Tech, Shahrood, Iran

4 صنعتی شاهرود-مهندسی مکانیک

5 دانشگاه صنعتی شاهرود

چکیده

در زمینه تولید قطره، تنها تعداد معدودی از آن‌ها به کنترل حلقه‌بسته اندازه میکروقطره به صورت آنلاین پرداخته‌اند. در مطالعه پیش‌رو، ابتدا یک میکروکانال جریان متمرکزشونده به روش فوتولیتوگرافی ساخته شد. سپس آب دو بار تقطیر با دبی ثابت به عنوان سیال فاز گسسته و روغن به عنوان سیال فاز پیوسته به وسیله دو پمپ سرنگی مجزا درون میکروکانال تزریق شدند و بدین ترتیب تولید قطرات آب در روغن صورت پذیرفت. سپس با استفاده از یک میکروسکوپ دیجیتالی سرعت بالا به همراه یک الگوریتم پردازش تصویر، قطر قطرات تولیدشده اندازه‌‏گیری شد. پس از ایجاد ارتباط بین میکروسکوپ، پمپ‌های سرنگی و کامپیوتر، طراحی یک فلودیگرام کنترلی صورت پذیرفت. همچنین به منظور کنترل قطر قطرات، دبی فاز پیوسته به عنوان پارامتر کنترلی انتخاب و با استفاده از کنترل‏کننده تناسبی-انتگرال‌گیر-مشتق‌گیر کنترل شد. در این مطالعه قطر قطرات تولیدشده در یک میکروکانال به صورت لحظه‏ای اندازه‌گیری و بازخورد آن دریافت شده‌است. نتایج عملی برای سه قطر مطلوب 100، 140 و 160 میکرومتر نشان داد که در هر سه حالت سیستم کنترلی توانست قطر قطره‏‌های در حال تولید را در مقدار مطلوب تنظیم کند. همچنین با تغییر پله‌‏ای دبی فاز گسسته، از عملکرد مطلوب کنترل‏کننده در زمان اغتشاش اطمینان حاصل شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Control of droplet size in a two-phase microchannel using PID controller: A novel experimental study

نویسندگان [English]

  • Sina Mottaghi 1
  • Mostafa Nazari 2
  • Mohsen Nazari 3
  • Naserodin Sepehry 4
  • Amir Mahdavi 5
1 MSc of mechanical engineering, Shahrood university of technology
2 Department of Mechanical Engineering, Shahrood University of Technology, Shahrood, Iran.
3 Shahrood University of Tech, Shahrood, Iran
4 صنعتی شاهرود-مهندسی مکانیک
5 Shahrood University of Technology
چکیده [English]

Precise droplet generation with controllable precise size is the target of this research. For this purpose, a flow focusing micro-channel is constructed using photolithography. Two syringe pumps are used, one for injecting discrete phase flow (DI water) and another for injecting continuous phase flow (oil). The Meros high speed camera is used for recording the image of droplets, and a fast image processing algorithm is used to calculate the size of the droplets. To regulate the size of the droplet, the PID controller is used due to its ease of implementation and robustness. The flow rate of the continuous phase flow is the control input and the size of the droplets is the output of the closed-loop system. Experimental tests are done by considering three desired droplet diameters, i.e. 100, 140 and 160 . To show the disturbance rejection characteristic of the designed closed-loop system, the flow rate of the discrete phase flow is changed stepwise. Due to this disturbance, the transient response of the system changed, but the controller attenuates this disturbance and regulates the system to the desired size. The experimental tests show that the designed closed-loop microfluidic system can generate droplets with desired precise size.

کلیدواژه‌ها [English]

  • Microchannel
  • active control
  • PID controller
  • two-phase flow
  • Micro-droplet
[1] S.-Y. Park, T.-H. Wu, Y. Chen, M.A. Teitell, P.-Y. Chiou, High-speed droplet generation on demand driven by pulse laser-induced cavitation, Lab on a Chip, 11(6) (2011) 1010-1012.
[2] E. Brouzes, M. Medkova, N. Savenelli, D. Marran, M. Twardowski, J.B. Hutchison, J.M. Rothberg, D.R. Link, N. Perrimon, M.L. Samuels, Droplet microfluidic technology for single-cell high-throughput screening, Proceedings of the National Academy of Sciences, 106(34) (2009) 14195-14200.
[3] J. Clausell-Tormos, D. Lieber, J.-C. Baret, A. El-Harrak, O.J. Miller, L. Frenz, J. Blouwolff, K.J. Humphry, S. Köster, H. Duan, Droplet-based microfluidic platforms for the encapsulation and screening of mammalian cells and multicellular organisms, Chemistry & biology, 15(5) (2008) 427-437.
[4] A. Huebner, M. Srisa-Art, D. Holt, C. Abell, F. Hollfelder, A. Demello, J. Edel, Quantitative detection of protein expression in single cells using droplet microfluidics, Chemical communications, (12) (2007) 1218-1220.
[5] L.S. Roach, H. Song, R.F. Ismagilov, Controlling nonspecific protein adsorption in a plug-based microfluidic system by controlling interfacial chemistry using fluorous-phase surfactants, Analytical chemistry, 77(3) (2005) 785-796.
[6] W. Li, H.H. Pham, Z. Nie, B. MacDonald, A. Güenther, E. Kumacheva, Multi-step microfluidic polymerization reactions conducted in droplets: The internal trigger approach, Journal of the American Chemical Society, 130(30) (2008) 9935-9941.
[7] Y.-H. Chang, G.-B. Lee, F.-C. Huang, Y.-Y. Chen, J.-L. Lin, Integrated polymerase chain reaction chips utilizing digital microfluidics, Biomedical microdevices, 8(3) (2006) 215-225.
[8] B.T. Lau, C.A. Baitz, X.P. Dong, C.L. Hansen, A complete microfluidic screening platform for rational protein crystallization, Journal of the American Chemical Society, 129(3) (2007) 454-455.
[9] L.-H. Hung, K.M. Choi, W.-Y. Tseng, Y.-C. Tan, K.J. Shea, A.P. Lee, Alternating droplet generation and controlled dynamic droplet fusion in microfluidic device for CdS nanoparticle synthesis, Lab on a Chip, 6(2) (2006) 174-178.
[10] Z.Z. Chong, S.B. Tor, A.M. Gañán-Calvo, Z.J. Chong, N.H. Loh, N.-T. Nguyen, S.H. Tan, Automated droplet measurement (ADM): an enhanced video processing software for rapid droplet measurements, Microfluidics and Nanofluidics, 20(4) (2016) 66.
[11] T. Thorsen, R.W. Roberts, F.H. Arnold, S.R. Quake, Dynamic pattern formation in a vesicle-generating microfluidic device, Physical review letters, 86(18) (2001) 4163.
[12] P. Garstecki, M.J. Fuerstman, H.A. Stone, G.M. Whitesides, Formation of droplets and bubbles in a microfluidic T-junction—scaling and mechanism of break-up, Lab on a Chip, 6(3) (2006) 437-446.
[13] S.-H. Tan, N.-T. Nguyen, L. Yobas, T.G. Kang, Formation and manipulation of ferrofluid droplets at a microfluidic T-junction, Journal of Micromechanics and Microengineering, 20(4) (2010) 045004.
[14] S.L. Anna, N. Bontoux, H.A. Stone, Formation of dispersions using “flow focusing” in microchannels, Applied physics letters, 82(3) (2003) 364-366.
[15] A.M. Gañán-Calvo, Generation of steady liquid microthreads and micron-sized monodisperse sprays in gas streams, Physical review letters, 80(2) (1998) 285.
[16] M.A. Herrada, A.M. Gañán-Calvo, Swirl flow focusing: A novel procedure for the massive production of monodisperse microbubbles, Physics of Fluids, 21(4) (2009) 042003.
[17] F. Dutka, A.S. Opalski, P. Garstecki, Nano-liter droplet libraries from a pipette: step emulsificator that stabilizes droplet volume against variation in flow rate, Lab on a Chip, 16(11) (2016) 2044-2049.
[18] K. Kang, S.H. Lee, H.S. Ryou, Nanoscale Microscale Thermophys. Eng. Nanoscale Microscale Thermophys. Eng. 10, 217-232, 2006, Nanoscale, 10 (2006) 217-232.
[19] R. Seemann, M. Brinkmann, T. Pfohl, S. Herminghaus, Droplet based microfluidics, Reports on progress in physics, 75(1) (2011) 016601.
[20] K.W. Oh, C.H. Ahn, A review of microvalves, Journal of micromechanics and microengineering, 16(5) (2006) R13.
[21] M. Simon, V. Bright, R. Radebaugh, Y. Lee, An analytical model for a piezoelectric axially driven membrane microcompressor for optimum scaled down design, Journal of Mechanical Design, 134(1) (2012).
[22] J. Luo, Y.Q. Fu, Y. Li, X. Du, A. Flewitt, A. Walton, W. Milne, Moving-part-free microfluidic systems for lab-on-a-chip, Journal of Micromechanics and Microengineering, 19(5) (2009) 054001.
[23] W. Zeng, I. Jacobi, D.J. Beck, S. Li, H.A. Stone, Characterization of syringe-pump-driven induced pressure fluctuations in elastic microchannels, Lab on a Chip, 15(4) (2015) 1110-1115.
[24] B.S. Hardy, K. Uechi, J. Zhen, H.P. Kavehpour, The deformation of flexible PDMS microchannels under a pressure driven flow, Lab on a Chip, 9(7) (2009) 935-938.
[25] K.W. Oh, K. Lee, B. Ahn, E.P. Furlani, Design of pressure-driven microfluidic networks using electric circuit analogy, Lab on a Chip, 12(3) (2012) 515-545.
[26] Y.J. Heo, J. Kang, M.J. Kim, W.K. Chung, Tuning-free controller to accurately regulate flow rates in a microfluidic network, Scientific reports, 6 (2016) 23273.
[27] J.B. Christen, A.G. Andreou, Design, fabrication, and testing of a hybrid CMOS/PDMS microsystem for cell culture and incubation, IEEE Transactions on Biomedical Circuits and Systems, 1(1) (2007) 3-18.
[28] E. Miller, M. Rotea, J.P. Rothstein, Microfluidic device incorporating closed loop feedback control for uniform and tunable production of micro-droplets, Lab on a Chip, 10(10) (2010) 1293-1301.
[29] Y. Kim, B. Kuczenski, P.R. LeDuc, W.C. Messner, Modulation of fluidic resistance and capacitance for long-term, high-speed feedback control of a microfluidic interface, Lab on a Chip, 9(17) (2009) 2603-2609.
[30] Y.J. Heo, J. Kang, W.K. Chung, Robust control for valveless flow switching in microfluidic networks, in:  2015 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS), IEEE, 2015, pp. 1982-1987.
[31] H. Fu, W. Zeng, S. Li, S. Yuan, Electrical-detection droplet microfluidic closed-loop control system for precise droplet production, Sensors and Actuators A: Physical, 267 (2017) 142-149.
[32] H. Kim, D. Luo, D. Link, D.A. Weitz, M. Marquez, Z. Cheng, Controlled production of emulsion drops using an electric field in a flow-focusing microfluidic device, Applied Physics Letters, 91(13) (2007) 133106.
[33] A.S. Basu, Droplet morphometry and velocimetry (DMV): a video processing software for time-resolved, label-free tracking of droplet parameters, Lab on a Chip, 13(10) (2013) 1892-1901.
[34] Z.Z. Chong, S.H. Tan, A.M. Gañán-Calvo, S.B. Tor, N.H. Loh, N.-T. Nguyen, Active droplet generation in microfluidics, Lab on a Chip, 16(1) (2016) 35-58.